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On-line HPLC for the determination of cephalosporin C and by-products in complex fermentation broths
Summary
Type:
HPLC
Application #:
103510
Catalog#:
720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection:
UV, 225nm, 0.1 AUFS
Conditions:
temperature 30°C
eluent: methanol - 14mM phosphate buffer pH 6.5 containing 3.5g/l tetrabutylammonium hydrogen sulphate (5:95)
flow rate 1ml/min
Authors:
Holzhauer-Rieger K
Source:
J Chromatogr 499 (1990) 609-615
Details
Legend: Fig. 3. Chromatograms of a calibration and a fermentation sample, left: standard solution, 2.38g/l MET, 0.4g/l DAC, 0.26g/l PEN N, 1.297g/l CPC, 1.32g/l MMBS, right: chromatogram of a CPC fermentati
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